Sarah Khalifa; Maha E Muhammad; Rasha A. Khairy MD; Hala N Hosni; Ahmed M Abd E
Volume 3, Issue 1 , March and April 2018, , Pages 13-18
Abstract
Aims: Supporting evidences have been proposed that tumor initiating cells or cancer stem cells (CSCs) are principally sharing into tumor progress and relapse. Aldehyde dehydrogenase 1A1 (ALDH1A1), a stem cell marker, has currently been implicated in multiple human malignancies including ovarian carcinomas. ...
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Aims: Supporting evidences have been proposed that tumor initiating cells or cancer stem cells (CSCs) are principally sharing into tumor progress and relapse. Aldehyde dehydrogenase 1A1 (ALDH1A1), a stem cell marker, has currently been implicated in multiple human malignancies including ovarian carcinomas. The aims of this study were to assess immunohistochemical (IHC) expression of ALDH1A1 in ovarian epithelial tumors, tracking stem cells during ovarian cancer development and its relation with the clinicopathological features of such tumors.
Materials and Methods: In this experimental study, 42 paraffin blocks of ovarian tumor cases were retrieved retrospectively from the department of pathology, faculty of medicine, Cairo University during January 2013 to January 2015. Ovarian tumor paraffin blocks included 14 benign cystadenomas 14 border line tumors and 14 carcinomas. IHC reactions were carried out by using ALDH1A1 monoclonal antibody. Cases were classified into two groups of low and high ALDH1A1 expression. The chi-squared test (χ2) was used and data analyzes by SPSS 22 software.
Findings: High ALDH1A1 expression was reported in 50.0% of benign cystadenomas, 50.0% of borderline tumors and 85.7% of ovarian carcinomas with significant positive association with ovarian carcinomas (p=0.025). In ovarian carcinomas, positive relationship was detected between high ALDHA1 expression and advancing tumor grades but it didn’t reach statistical significance (p=0.054), no any significant relations were detected between ALDH1A1 immunohistochemical expression and age of patients, the documented size, laterality, histologic types and FIGO stage in all tumors (p>0.05).
Conclusion: ALDH1A1 is a potential biomarker for detecting CSCs in ovarian carcinomas and a prognostic marker. Also, it may act as a target for future therapy.
Waleed El-Khayat; Fatmafaisal Ibraheem; Amal Mohsen Ahmed; Ashraf Abdelrahman Ramadan; Sara E Khalifa; Mohammed Faisal Ebrahim
Volume 2, Issue 2 , May and June 2017
Abstract
Background: Abnormal uterine bleeding is a very common gynecological problem, especially in perimenopausal women. Intracavitary lesions have been reported in more than 40% of the referred patients.
Objectives: To compare the diagnostic accuracy of 3-dimensional sonohysterography (3D-SHG) versus hysteroscopy ...
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Background: Abnormal uterine bleeding is a very common gynecological problem, especially in perimenopausal women. Intracavitary lesions have been reported in more than 40% of the referred patients.
Objectives: To compare the diagnostic accuracy of 3-dimensional sonohysterography (3D-SHG) versus hysteroscopy in the evaluation of uterine cavity in patients with perimenopausal bleeding and suspected intracavitary lesions, to evaluate the correlations between these findings and histopathological results, and to determine if 3D-SHG is superior or comparable to hysteroscopy in the diagnosis of these lesions.
Methods: A total of 50 women with perimenopausal bleeding and suspected intracavitary lesions, detected by either 2D transvaginal ultrasound or hysterosalpingography, were subjected to 3D saline-infusion sonohysterography (3D-SIS), office hysteroscopy, subsequent surgical procedures (i.e., fractional curettage, hysterectomy, polypectomy, or myomectomy), and finally histopathological examinations.
Results and Conclusions: Based on the findings, 3D-SIS is comparable to hysteroscopy in the diagnosis of intracavitary lesions in terms of sensitivity, specificity, positive predictive value, negative predictive value, and overall accuracy
