Leili Bargi; Changiz Ahmadizadeh
Volume 4, Issue 2 , May and June 2019, , Pages 62-68
Abstract
Background & Objective: Cervical cancer is the second most prevalent cancer in women worldwide and is one of the leading causes of deaths in women with cancer in developing countries. The most well-known cause of this cancer is being infected with human papillomavirus (HPV). This study aimed ...
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Background & Objective: Cervical cancer is the second most prevalent cancer in women worldwide and is one of the leading causes of deaths in women with cancer in developing countries. The most well-known cause of this cancer is being infected with human papillomavirus (HPV). This study aimed at evaluating the concurrent methylation pattern of the human papillomavirus genome in studying the DAPK1 gene as a diagnostic biomarker in cervical cancer.
Materials & Methods: This study was approved by the Ethics Committee of the Islamic Azad University of Tabriz under the following ethical code, i.e., IR.IAU.TABRIZ.REC1398.001.This case-control study was conducted on 150 paraffin-embedded samples (75 marginal samples and 75 cervical cancer tissue samples) taken from women with cervical cancer. After extracting the total DNA, spectrophotometry and electrophoresis were performed on agarose gels to determine the quantity and quality of the extracted DNA. Changes in the DAPK1 gene methylation pattern were examined using High Resolution Melt Analysis Curve Method.
Results: The changes in theDAPK1 gene expression in the cervical tumor tissues increased compared to the marginal tissues, indicating a statistically significant difference (P < /em><0/0001).
Conclusion: The results of the current study showed that the DAPK1 gene can be introduced as a possible prognosis for cervical cancer diagnosis and treatment. However, more extensive studies with more samples are needed to be carried out.
Malihe Hasanzadeh; Shahnaz Ahmadi
Volume 1, Issue 2 , September and October 2016
Abstract
Background: Human papillomavirus (HPV) is thought to be the most common sexually transmitted viral disease. This infection continues to be an important topic. One of the most important conferences on human papillomavirus infection and related cancers is EUROGIN. The program also includes state of the ...
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Background: Human papillomavirus (HPV) is thought to be the most common sexually transmitted viral disease. This infection continues to be an important topic. One of the most important conferences on human papillomavirus infection and related cancers is EUROGIN. The program also includes state of the art science on anogenital and head and neck cancer, inspiration, cooperation, and forums to share expertise and learn from leading experts in the field.
Methods: We reported an abstract of important articles and researches presented in this congress.
Results: HPV had rolled in oropharyngeal cancer. KI67/P16 is important for deciding on treatment of patients with HPV high-risk positive. Methylation can be used in the management of HPV high-risk patients. 9-valent HPV vaccination can prevent different anogenital cancers.
Conclusions: HPV has important role in different cancers. HPV vaccination can prevent a variety of anogenital cancers related to HPV.
