Obstetrics and Gynecology
Dhai Abdulalazize Rashid; Fadia Jassim Alizzi
Volume 8, Issue 3 , May and June 2023, , Pages 233-239
Abstract
Background & Objective: Infertility that cannot be explained by anovulation, poor sperm quality, tubal disease, or any other recognized cause of infertility is referred to as unexplained infertility. The aim of this study is to assess the correlation between integrin alpha-v beta-3 expression, ...
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Background & Objective: Infertility that cannot be explained by anovulation, poor sperm quality, tubal disease, or any other recognized cause of infertility is referred to as unexplained infertility. The aim of this study is to assess the correlation between integrin alpha-v beta-3 expression, endometrial thickness and subendometrial blood flow resistance index in unexplained infertility.Materials & Methods: The study carried out on Kamal – Al-Samurai fertility center in the period from October 2020 - September 2021. Six days after detecting urinary Luteinizing Hormone (LH) surge, all the women were asked to come to do two-dimensional (2D) transvaginal ultrasound to assess endometrial thickness and subendometrial blood flow color Doppler resistance index and endometrial samples was taken and examined immunohistochemically to detect alpha-v beta-3 integrin. The cut-off value of integrin and subendometrial blood flow resistance index, sensitivity and specificity were calculated by applying Receiver Operative Characteristics (ROC) curve.Results: The endometrial thickness was lower and resistance index was higher in case group (P < 0.001). The expression of integrin alpha-v beta-3 in infertile group was significantly low (P < 0.001) with significant positive correlation between integrin score and endometrial thickness (r= -0.708 & P < 0.001) and significant negative correlation between integrin score and subendometrial resistance index (r= -0.786 & P <0.001).Conclusion: Alpha-v beta-3 integrin expressions and endometrial thickness are reduced significantly in mid-luteal phase while the subendometrial blood flow color Doppler resistant index significantly increased, and using them together can possibly be used as a diagnostic predictor of unexplained infertility.
Reproductive Medicine
Shamim Ashrafi Mahabadi; Farzaneh Tafvizi
Volume 5, Issue 4 , December 2020, , Pages 149-158
Abstract
Background and Objectives: Soluble Leptin Receptor (sOB-R) is the most important leptin-binding protein that can affect the level of active biological leptin. The Gln223Arg polymorphism in the leptin receptor gene is associated with obesity, which can be a factor for infertility. The purpose of ...
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Background and Objectives: Soluble Leptin Receptor (sOB-R) is the most important leptin-binding protein that can affect the level of active biological leptin. The Gln223Arg polymorphism in the leptin receptor gene is associated with obesity, which can be a factor for infertility. The purpose of this study was to evaluate the sOB-R levels in women with unexplained infertility and to investigate the effect of leptin receptor (LEPR) gene polymorphism on sOB-R serum level.Methods: One hundred and two women with unexplained infertility and 112 fertile women were studied in this case-control study. The subjects in both groups had a normal hormonal profile with the age below 40 years. The sOB-R levels were measured using ELISA method.Results: There was a 2-fold decrease in sOB-R level in the infertile group compared to the control group (P < /em>=0.001). Although sOB-R levels were lower in overweight and obese infertile subjects, this difference was not significant. However, this difference was significant compared to the control group. There was a direct correlation between BMI, age, and infertility. Although sOB-R levels in Arg/Arg genotypes were higher in the infertile group than Gln/Gln and Arg/Gln genotypes, they were not statistically significant. A significant decrease was observed in sOB-R levels of Gln/Gln and Arg/Gln genotypes in the infertile group compared to the control.Conclusion: There was a reverse correlation between sOB-R levels and unexplained infertility. It seems that by a decrease in serum sOB-R, the serum leptin levels increase which can enhance leptin inhibitory effects in infertility.
