Reproductive Medicine
shayamaa Abd Hasan; Basma Salman Abdul Hammed; Qasim Mohammed Banjah
Articles in Press, Accepted Manuscript, Available Online from 19 November 2023
Abstract
Primary infertility affects a significant proportion of couples and is often attributed to female factors. Obesity, which is associated with increased levels of the hormone leptin, is a known risk factor for female infertility. This cross-sectional study aimed to investigate the association between BMI ...
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Primary infertility affects a significant proportion of couples and is often attributed to female factors. Obesity, which is associated with increased levels of the hormone leptin, is a known risk factor for female infertility. This cross-sectional study aimed to investigate the association between BMI and leptin levels in primary infertile females at Al-Nuaman Hospital and Al-Salama private hospital from September 2022 to March 2023. A total of 100 females were enrolled, and their BMI and serum leptin levels were measured. The participants were divided into four groups: Group 1 (normal weight, fertile), Group 2 (overweight infertile), Group 3 (obese infertile), and Group 4 (severely obese infertile) women, each consisting of 25 participants, categorized based on their BMI: normal BMI (18.5–24.9 kg/m²), overweight BMI (25–29.9 kg/m²), obesity BMI (≥30 kg/m²), and severely obese BMI (35-40 kg/m²). The mean leptin levels were compared between the four groups using ANOVA, and the association between BMI and leptin levels was assessed using Pearson's correlation coefficient. The study revealed a highly significant correlation between primary infertility and the parameters (BMI and leptin) (P < 0.001) and showed a positive correlation between leptin and BMI. It was shown that primary infertile females with a high BMI have elevated serum leptin levels. This underscores the importance of weight management in addressing primary female infertility.
Obstetrics and Gynecology
Zinah Hammad; Ban J. Edan
Volume 8, Issue 2 , March and April 2023, , Pages 167-172
Abstract
Background & Objective: One of the worldwide healthcare problems is Diabetes mellitus (DM), and the prevalence of this disease increases steadily, particularly in pregnancy. The several metabolic disorders in the pregnancy were revealed by the placenta. The pathogenesis of diabetes mellitus ...
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Background & Objective: One of the worldwide healthcare problems is Diabetes mellitus (DM), and the prevalence of this disease increases steadily, particularly in pregnancy. The several metabolic disorders in the pregnancy were revealed by the placenta. The pathogenesis of diabetes mellitus is associated with hyperglycemia, insulin resistance, and oxidative stress, and it negatively impacts the mother-placenta-fetus system. This research aims to compare the amount of leptin expression, as well as its score and intensity, in the placentas of women with diabetes with those of healthy controls.Materials & Methods: In this work, we used a case-control methodology. Thirty women were diagnosed with diabetes and were placed in one group, while the other 40 served as a comparison. Primary monoclonal antibodies were used for the immunohistochemical analysis of leptin (Abcam, UK).Results: The patients group showed the greatest percentage (23%) of placental expression of leptin. The expression of leptin was high in around 53% of the patient population. The intensity of leptin expression was significantly higher in DM patient than control groups (P>0.05). About 77% of patients group had moderate intensity of leptin expression, while 37.5% of control group had no leptin expression.Conclusion: Diabetes mellitus was related with alterations in expressions of leptin as metabolic placental factor. Leptin may affect the pregnancy diverse disorders and pregnancy outcome.
Reproductive Medicine
Shamim Ashrafi Mahabadi; Farzaneh Tafvizi
Volume 5, Issue 4 , December 2020, , Pages 149-158
Abstract
Background and Objectives: Soluble Leptin Receptor (sOB-R) is the most important leptin-binding protein that can affect the level of active biological leptin. The Gln223Arg polymorphism in the leptin receptor gene is associated with obesity, which can be a factor for infertility. The purpose of ...
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Background and Objectives: Soluble Leptin Receptor (sOB-R) is the most important leptin-binding protein that can affect the level of active biological leptin. The Gln223Arg polymorphism in the leptin receptor gene is associated with obesity, which can be a factor for infertility. The purpose of this study was to evaluate the sOB-R levels in women with unexplained infertility and to investigate the effect of leptin receptor (LEPR) gene polymorphism on sOB-R serum level.Methods: One hundred and two women with unexplained infertility and 112 fertile women were studied in this case-control study. The subjects in both groups had a normal hormonal profile with the age below 40 years. The sOB-R levels were measured using ELISA method.Results: There was a 2-fold decrease in sOB-R level in the infertile group compared to the control group (P < /em>=0.001). Although sOB-R levels were lower in overweight and obese infertile subjects, this difference was not significant. However, this difference was significant compared to the control group. There was a direct correlation between BMI, age, and infertility. Although sOB-R levels in Arg/Arg genotypes were higher in the infertile group than Gln/Gln and Arg/Gln genotypes, they were not statistically significant. A significant decrease was observed in sOB-R levels of Gln/Gln and Arg/Gln genotypes in the infertile group compared to the control.Conclusion: There was a reverse correlation between sOB-R levels and unexplained infertility. It seems that by a decrease in serum sOB-R, the serum leptin levels increase which can enhance leptin inhibitory effects in infertility.
