Iranian Society of Gynecology Oncology


1 Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.

2 Thalassemia & Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 MD, professor of hematopathology, Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

4 Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


Background: Recurrent pregnancy loss (RPL) is the occurrence of three or more miscarriages before the 20th week of pregnancy. Thrombophilia factors are one of the main causes of RPL.
Methods: This retrospectively study was performed on women with more than two miscarriages. 620 patients’ documents with pregnancy loss were investigated. Based on number of pregnancy loss, the women divided to a control group with less than three miscarriages (212) and RPL group (180). Cytogenetics analysis and thrombophilia panel (MTHFR 677 C⁄T/ FV Leiden G1691A/Prothrombin G20210A (FII), ACE I/D, PAI1) were performed for all patients.
Result: In the analysis between Control and RPL groups, none of the studied polymorphisms (MTHFR 677 C⁄T /Factor V Leiden /Prothrombin G20210A/ ACE I/D/ PAI-1) showed a significant relationship (P-value ˃ 0.05). Cytogenetic analysis showed 2 numerical and 9 structural abnormalities among both groups. Statistical analysis indicated significant association between number of abortion and age (P value= 0.005, r =0.139). We even realized that there was significant relationship between polymorphism number and recurrent number of abortion (P value= 0.018, r = 0.6).
Conclusion: We showed that polymorphisms analysis for thrombophilia factors is more precious test than cytogenetics analysis for RPL detection due to frequency in pregnant woman. We even indicated that no association was found between thrombophilia polymorphisms in Control and RPL groups. This means that screening for Factor V Leiden, prothrombin G20210A, MTHFR C677T, ACE I/D and PAI-1 and cytogenetic analysis in patients with a history of RPL is not recommended.



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